Serial effects of a single intravenous dose, 25 μg/kg (○) or 250 μg/kg (•) of PEG-rmMGDF administered on day 0 on the percentage of large platelets defined by flow cytometry to be larger than 99% of platelets of control mice. A major increase in the percentage of large platelets was noted on day 2 after PEG-rmMGDF, with peaks occurring on day 3 and days 3 to 4 after the 25 and 250 μg/kg doses, respectively. Each data point represents the mean ± SEM of the percentage of large platelets, for the 25 μg/kg dose, n = 4 to 7 mice per time point for the MGDF group and n = 12 for controls, and for the 250 μg/kg dose, n = 5 to 6 mice per time point for the MGDF group and n = 15 for controls. Data from control mice were pooled to represent the day 0 data point.