Fig. 6.
IL-6 protein secretion by PMNs after (A) cross-linking FcγRI or FcγRII and after (B) rhG-CSF stimulation. IL-6 concentrations (in picograms per milliliter) in supernatants after incubation of PMNs cultured in vitro for 1 hour (▪) or 4 hours (▧). IL-6 concentrations were measured by ELISA. Purified PMNs were obtained from three normal donors (A) and from three patients who had been treated with rhG-CSF in vivo (B). Each value depicts the mean ± SEM of three separate experiments. IL-6 concentrations were measured after incubation of PMNs under the conditions listed. CL, cross-linker goat antimouse IgG and IgMF(ab′)2; anti-FcγRI Ab, 32.2 (Fab′)2; anti-FcγRII Ab, IV.3 Fab′.
