The effect of cross-linking FcγRI or FcγRII on total cellular IL-6 protein production by PMNs. Flow cytometry histograms depict total intracellular IL-6 protein in freshly isolated PMNs from a normal donor (A through C) or PMNs from a patient treated with rhG-CSF in vivo (D through F). In each panel, the solid histogram presents isotype control staining. In (A) and (D), the histograms depict fluorescence staining of freshly isolated PMNs in media (—) and staining of PMNs after exposure to cross-linker alone (···). In (B) and (E), the histograms depict fluorescence staining of PMNs after incubation with media (—) or with (Fab′)2 32.2 anti-FcγRI (···). In (C) and (F), the histograms depict fluorescence staining of PMNs after incubation with media (—) or with Fab′ IV.3 (···). In (B), (C), (E), and (F), the bold line depicts staining after cross-linking anti-FcγRI or anti-FcγRII as listed above. This experiment is representative of four virtually identical experiments.
