Fig. 1.
Fig. 1. Family tree for the Rhnull donor Y.T., showing inheritance of the RH50 allele containing the G836A mutation. PCR products were prepared from genomic DNA using primers D and C that span nt 820 to 908. Primer D has a 12-base tail added. The 101-bp PCR product obtained from each family member was digested withMnlI, and the fragments were analyzed on agarose gel. Products containing the G836 sequence are cut into 22- and 79-bp fragments, whereas those with A836 are uncut. The polymorphism at nt 836 (A or G) is indicated below each family member.

Family tree for the Rhnull donor Y.T., showing inheritance of the RH50 allele containing the G836A mutation. PCR products were prepared from genomic DNA using primers D and C that span nt 820 to 908. Primer D has a 12-base tail added. The 101-bp PCR product obtained from each family member was digested withMnlI, and the fragments were analyzed on agarose gel. Products containing the G836 sequence are cut into 22- and 79-bp fragments, whereas those with A836 are uncut. The polymorphism at nt 836 (A or G) is indicated below each family member.

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