Fig. 9.
![Fig. 9. Effect of HNF-4 on transient expression of reporter constructs in P19 cells. P19 cells were cotransfected with the reporter plasmids shown in the figure and HNF-4 expression plasmid [pLEn(M4)LPHNF-4] to determine if HNF-4 activates the Epo enhancer in P19 cells. In (A), combination of LDHA promoter (LP) and Epo enhancer (En); in (B), combination of human Epo promoter (0.2k) and En. Transient expression of reporter constructs in 21% and 2% oxygen was assayed. The ratio of HNF-4 expression plasmid versus reporter plasmid is shown. Hypoxic inducibility (the ratio of Luc activity in 2% oxygenv 21% oxygen) is indicated. Each value is the mean ± SD of triplicate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/4/10.1182_blood.v91.4.1185/4/blod4040509.jpeg?Expires=1730477690&Signature=QvABo9gHf3cTRDhSF4BdGLShywk4aIIDaFvdnUPY9gxv2iSrLuqXosubZ1zVbFdu7Os-PvAMi6Nf~7aXVEC4Y78Z~TmGyJ27TkDsVk9CbCAYN78TnMgLVC3~9-RhSco6-JTIkUFvTAsIRyNGBoj6SC7SNemMarfXV8ZhPDH53ptzt-w~WlhoL0dp6ce3Bxs7qPew3G0rlsGxyXkBK62HEFHNM1CDQxo8OtlqhsTSuTLh5VXOsvyPPZfLpEQBpTWt0lVr-F4e6Ebis2jYKI1PqqKrfrybFveyOPfNQYwZhrIPpUCgSuVKJAIimymWN0~T9nH-vIx~TXptEo5WALmRdg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of HNF-4 on transient expression of reporter constructs in P19 cells. P19 cells were cotransfected with the reporter plasmids shown in the figure and HNF-4 expression plasmid [pLEn(M4)LPHNF-4] to determine if HNF-4 activates the Epo enhancer in P19 cells. In (A), combination of LDHA promoter (LP) and Epo enhancer (En); in (B), combination of human Epo promoter (0.2k) and En. Transient expression of reporter constructs in 21% and 2% oxygen was assayed. The ratio of HNF-4 expression plasmid versus reporter plasmid is shown. Hypoxic inducibility (the ratio of Luc activity in 2% oxygenv 21% oxygen) is indicated. Each value is the mean ± SD of triplicate experiments.
