Effect of flavopiridol on growth of SUDHL4, HL60, MOLT4, K562, and PC3 cells. Cells (2 × 10⁴ cells/mL) were plated into each well of a six-well plate. After 24 hours, cells were treated in triplicate either with vehicle or with 50, 100, and 500 nmol/L of flavopiridol for 48 hours. Cell growth was determined by counting live (Trypan Blue excluded) and dead (Trypan Blue stained) cells on a hemacytometer. (A) Represents growth inhibition as percent of control for SUDHL4 (○), HL60 (□), MOLT4 (▵), K562 (▿), and PC3 (◊) cells after 48 hours drug exposure. (B) Presents the results as percent of total live cells (open bar) and dead cells (hatched bar) after 500 nmol/L flavopiridol exposure for 48 hours. The experiments represent the mean of three determinations ± SD.