Intracellular localization of BCR/ABL proteins. (Top panel) Membrane (m) and cytosolic (c) BCR/ABL, p120 GAP, and transferrin receptor (CD71) were analyzed in BCR/ABL (wild-type and ▵SH3) transfectants by SDS-PAGE followed by Western blotting with anti-ABL, anti-GAP, and anti-CD71 MoAbs. As a control, the same proteins were also detected in total cell lysates. Results are representative of three different experiments. (Bottom panel) 32Dcl3 cells expressing wild-type (A) or ▵SH3 mutant (B) BCR/ABL were incubated with anti-ABL followed by FITC-conjugated goat antirabbit antibody and examined by confocal microscopy. Results are representative for three individual clones from each group (original magnification × 800).