Transactivation of a minimal TK promoter containing the murine c-mpl GATA-binding site. Jurkat cells were transfected with either pTKLuc/m c-mpl containing a wild-type GATA-binding site, or pTKLuc/m c-mpl containing a mutated GATA-binding site, bp −85 to −80, with and without 10 μg of pEF-hGATA-1. The results are expressed as the average fold increase in ability on addition of pEF-hGATA-1. The error bars represent the standard error of the mean of at least four experiments. Luciferase assay was normalized to β-galactosidase produced by a cotransfected CMV-β Gal plasmid.