Fig. 2.
Effect of D-glucose on superoxide production by human neutrophils. Neutrophils (106) were treated with 100 ng/mL PMA in the presence of increasing concentrations of D-glucose for 20 minutes at 37°C and SOD-inhibitable cytochrome c reduction determined. Result are the average of two experiments involving duplicate determinations made in the presence (•) or absence (○) of 1.4 mmol/L Ca2+ and Mg2+. Control absorbances at A550 (no glucose) were 0.722 ± 0.19 and 0.605 ± 0.017, respectively and in the presence of SOD, 0.376 ± 0.007 and 0.364 ± 0.013, respectively.
