Fig. 1.
Fig. 1. Effect of PMA on superoxide production by neutrophils and on [3H]-2–DOG uptake. (A) SOD-inhibitable cytochrome c reduction. Neutrophils (106) were treated with 100 ng/mL PMA for increasing times in the presence and absence of SOD. Control absorbance (A550 + SOD) was 0.364 ± 0.009. (B) [3H]-2–DOG uptake determined over 3-minute intervals at the times indicated after exposure to PMA. Each value represents the mean of duplicate determinations obtained from two separate experiments. [3H]-2–DOG uptake at zero time was 1619 ± 41 cpm.

Effect of PMA on superoxide production by neutrophils and on [3H]-2–DOG uptake. (A) SOD-inhibitable cytochrome c reduction. Neutrophils (106) were treated with 100 ng/mL PMA for increasing times in the presence and absence of SOD. Control absorbance (A550 + SOD) was 0.364 ± 0.009. (B) [3H]-2–DOG uptake determined over 3-minute intervals at the times indicated after exposure to PMA. Each value represents the mean of duplicate determinations obtained from two separate experiments. [3H]-2–DOG uptake at zero time was 1619 ± 41 cpm.

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