Dendritic cells are generated by stimulation of CD34⁺/CD86⁺ cells with TNF-α, GM-CSF, and SCF. CD34⁺/CD86⁻ (A and C) and CD34⁺/CD86⁺ (B and D) cells were cultured in the presence of TNF-α, GM-CSF, and SCF for 9 days. Cells were either Wright-Giemsa–stained and photographed at 100× amplification using oil immersion (A and B) or cells were photographed at 20× amplification by phase contrast (C and D). CD34⁺/CD86⁻ precursors generated a heterogeneous population of cells. In contrast, CD34⁺/CD86⁺ cells generated predominantly cells with dendritic morphology.