CD34⁺/CD86⁺ cells cultured in TNF-α, GM-CSF, and SCF acquire the surface phenotype of dendritic cells. After 14 days of culture in TNF-α, GM-CSF, and SCF, CD34⁺/CD86⁻ and CD34⁺/CD86⁺-derived cells were stained and analyzed by three-color microfluorimetry.