Fig. 3.
![Fig. 3. UV/visible spectrum of F417S recombinant human ferrochelatase. The spectrum of purified recombinant F417S ferrochelatase in elution buffer7 was obtained with a Varian 219 spectrophotometer. Not present are characteristic features resulting from the [2Fe-2S] cluster, which are normally present at 460 nm, 420 nm, and 325 nm in spectra from the wild-type protein.14 A minor absorbance peak at 410 nm is attributed to small amounts of residually bound porphyrin.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/10/10.1182_blood.v91.10.3980/3/blod41005003x.jpeg?Expires=1724226066&Signature=0EJidfoRtcdCgs1GeaRQBmOpVzP6t4cVNRx1ml1tlbNAPuYQl3JVccqYnbcUCy2qNQsmzZmyJJa6amAwdWGIZCEBrFDzmquhtLUeJpC1VKLdGtnBH3dF71FeervO8D1BajNdpSLE~YmWpJkcztf3XQsLPClxJbAhslYiVvvzyfBeCzjW9dW1Raj0fESJ8o9VeQ7wOqsPtm-yH3FgMGEnlOLN6q3iTBJelBJ6rYQMC6cMp2GvVzi20ZEj6Jr6vbM8Eal5K-VPByhWqOdLzzupKxR65ZU7Ekw2rTrMc-gUQnMskvU0rLzL-ysG0YDAE1aUli8-1fFQGjYTNZgz08Zaeg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
UV/visible spectrum of F417S recombinant human ferrochelatase. The spectrum of purified recombinant F417S ferrochelatase in elution buffer7 was obtained with a Varian 219 spectrophotometer. Not present are characteristic features resulting from the [2Fe-2S] cluster, which are normally present at 460 nm, 420 nm, and 325 nm in spectra from the wild-type protein.14 A minor absorbance peak at 410 nm is attributed to small amounts of residually bound porphyrin.
