Fig. 5.
![Fig. 5. Kinetics of [3H]-lyso-PI uptake. Transfer of [3H]-lyso-PI from donor vesicles to the cell membrane of lymphocytes was measured by scintillation counting of the incubation medium from EGTA-containing samples. The 100% value was obtained by measuring an aliquot of solution of 11.25 μmol/L [3H]-lyso-PI (concentration of substrate added to the cells). The kinetics of spontaneous uptake of the lipid by the cells shows that its concentrations in the medium plateaued at about 25% within 5 minutes. Beyond this time, the rate of lyso-PI transfer was evidently reduced. Data are the mean ± SD of at least 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/10/10.1182_blood.v91.10.3833/3/blod41041005y.jpeg?Expires=1724274576&Signature=2CX8vpt6O9viyL6vvg~198ckB2~UqoS3N4M8yTRsE7qipIxR4wDwD3wom5hskcqne8yEbteg-768au3mm00aXwGLxHZKHLe955NfyA0wZ8GyOHfSao3vpM~Hku882lCSLimQ4GjFPZ~4HjClYWjlNAMBW5Y2MRnlrkRbF7bCtvdsskFwumYioNQKxQLksvhqIRIpxzUVwJ90ISCUSfvhPcKFC7NtOKtOXtlqCDe7xRnVBi7ww2PsNP0JdVaYmp6K1Cri~d7NcawfBMWcvROi2Lokrr0C6-vX0xaiopBx7U1jhXGNYwoQ0wZ2yx9vUR5Nu27WXqormQIra7x-5sdUiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Kinetics of [3H]-lyso-PI uptake. Transfer of [3H]-lyso-PI from donor vesicles to the cell membrane of lymphocytes was measured by scintillation counting of the incubation medium from EGTA-containing samples. The 100% value was obtained by measuring an aliquot of solution of 11.25 μmol/L [3H]-lyso-PI (concentration of substrate added to the cells). The kinetics of spontaneous uptake of the lipid by the cells shows that its concentrations in the medium plateaued at about 25% within 5 minutes. Beyond this time, the rate of lyso-PI transfer was evidently reduced. Data are the mean ± SD of at least 3 separate experiments.
