Fig. 2.
Effect of shear rate and vWF on PAC-1 and S12 epitope expression. Washed platelets exposed to different shear rates were incubated with PAC-1 directed to activated αIIbβ3 or S12 to P-selectin. The percentage of positive platelets was assessed by flow cytometry by incubating the platelets with an FITC-conjugated secondary antibody. Means ± SEM were calculated from three experiments performed in duplicate. Platelets were exposed to different shear rates in the absence (•) or in the presence of 10 μg/mL vWF (▪). (A) PAC-1 expression. (B) S12 expression. Note differences in the scale of the y-axis.
