Multimer analysis of recombinant expressed vWF mutant missense mutations. Recombinant vWF was immunoprecipitated from conditioned media of transfected COS-7 cells, resolved by SDS/agarose gel electrophoresis and detected with ¹²⁵I–anti-vWF Ab as described in Materials and Methods. Pictured are autoradiograms of recombinant vWF (14 ng) containing the point mutation from Family A (F606I), Family B (I662F), wt recombinant vWF (WT) or immunoprecipitated conditioned medium from mock transfected cells (Mock).