Fig. 3.
Fig. 3. Reduced induction of p32/p30 phosphorylation from the EpoR mutant H/Y343F that was defective in Ras but not Jak2 activation. DA3 cells expressing the EpoR mutant H/Y343F were deprived of growth factors and then stimulated with IL-3 or Epo for various times. Cell lysates were prepared and analyzed by SDS-PAGE/Western blotting with antibodies as indicated.

Reduced induction of p32/p30 phosphorylation from the EpoR mutant H/Y343F that was defective in Ras but not Jak2 activation. DA3 cells expressing the EpoR mutant H/Y343F were deprived of growth factors and then stimulated with IL-3 or Epo for various times. Cell lysates were prepared and analyzed by SDS-PAGE/Western blotting with antibodies as indicated.

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