SHP-1-C induced hyperphosphorylation of p32/p30 but had no marked effect on the phosphorylation of EpoR and Jak2 and c-myc expression. Total cell lysates (TCL) were prepared from D/V and D/C cells stimulated with Epo for various times. The lysates were analyzed directly by SDS-PAGE/Western blotting or used in immunoprecipitation with antibodies as indicated. The positions of hyperphosphorylated proteins of approximately 30, 32, 53, 56, 104, and 150 kD and protein size markers (in kilodaltons) are indicated.