Fig. 6.
![Fig. 6. Simultaneous determination of apoptosis (ISEL) and DNA content (Hoechst 33324) in B-CLL CD19+ lymphocytes after 24-hour treatment in vitro with 3 μmol/L fludarabine (patient No. 5, group 1). B-CLL cells are mainly in the G0/1 cell cycle phases when they become positive for ISEL. Among the CD19+/ISEL+ lymphocytes, cells with diploid DNA content (early apoptotic cells, region [reg] 1) can be distinguished from cells with subdiploid DNA content (late apoptotic cells, reg 2).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/5/10.1182_blood.v91.5.1742/3/blod4052306.jpeg?Expires=1722785369&Signature=TeakJMgfyyCPazMVwDWe5Gr~FX5sOzX6ZOzKQAWIa93VDEfYd0lpB~mbaKVRU2~QOBN3Z2c9Ye~xY-k8y9Xz0ZXD0rKf1rnSGPAUnV0t7qE~QZdEWJrNVTbSvRLSOkRrqlGtqMrdmHARFhq9IKOG0h-2-Bu-eGaslYAu6XSQtppTSEPE2QJEfxy634Bo2OangQ1gjYWNlUufkGP2DG402v4-EX6O~DeQbm9Wsu14oWMDAGXOPA6WLo6uqgEZxG8zjXGqgnve5NLL2lBURR7rq6Ypb6LB9g7w0eBKw9VrKi2F~IsYqm451QpBIKxFmAeB6WlUjaUtdDgPjybx5m781Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Simultaneous determination of apoptosis (ISEL) and DNA content (Hoechst 33324) in B-CLL CD19+ lymphocytes after 24-hour treatment in vitro with 3 μmol/L fludarabine (patient No. 5, group 1). B-CLL cells are mainly in the G0/1 cell cycle phases when they become positive for ISEL. Among the CD19+/ISEL+ lymphocytes, cells with diploid DNA content (early apoptotic cells, region [reg] 1) can be distinguished from cells with subdiploid DNA content (late apoptotic cells, reg 2).
