Association of RAFTK with PI-3 kinase in THP1 cells. THP1 cells (20 × 10⁶) were stimulated with either (A) CSF-1/M-CSF (1,000 U/mL) or (B) LPS (2 μg/mL) for the indicated time periods. Cell lysates prepared in RIPA buffer were subjected to immunoprecipitation with anti-RAFTK antibody. Anti-RAFTK immunoprecipitates were resolved by 7.5% SDS-PAGE, transferred to nitrocellulose membranes, and immunoblotted with anti–PI-3 kinase antibody. TCL, total cell lysates.