L-selectin transfectants roll on an HL-60 lysate partially purified by WGA affinity chromatography. Nontransfected 300.19 cells, 300.19 L-selectin, or 300.19 P-selectin transfectants (1 × 106/mL) were infused through a parallel plate flow chamber at a wall shear stress of 0.64 dyne/cm2 in which a dilution of an HL-60 lysate partially purified by WGA affinity chromatography was adsorbed to the lower wall of the chamber. L-selectin and P-selectin–dependent adhesion were shown by incubation of L-selectin transfectants with MoAb DREG56 (10 μg/mL) and P-selectin transfectants with MoAb G1 (10 μg/mL). PSGL-1–dependent adhesion was shown by treating the adsorbed lysate with MoAb PL1 (10 μg/mL). Mean ± SEM of bound cells/mm2 in multiple fields from two to three independent experiments.
