Fig. 4.
Fig. 4. FucT-VII/PSGL-1 transfectants roll on L-selectin. K562-FucT-VII and K562-FucT-VII/PSGL-1 transfectants (0.5 × 106/mL) were infused through a parallel plate flow chamber at a wall shear stress of 1 dyne/cm2 in which purified human L-selectin (1:5; 170 sites/μm2) or P-selectin (1:60; 270 sites/μm2) were adsorbed to the lower wall of the chamber. Nontransfected K562 cells did not bind to L-selectin or P-selectin. Mean ± SEM of bound cells/mm2 in multiple fields from three independent experiments.

FucT-VII/PSGL-1 transfectants roll on L-selectin. K562-FucT-VII and K562-FucT-VII/PSGL-1 transfectants (0.5 × 106/mL) were infused through a parallel plate flow chamber at a wall shear stress of 1 dyne/cm2 in which purified human L-selectin (1:5; 170 sites/μm2) or P-selectin (1:60; 270 sites/μm2) were adsorbed to the lower wall of the chamber. Nontransfected K562 cells did not bind to L-selectin or P-selectin. Mean ± SEM of bound cells/mm2 in multiple fields from three independent experiments.

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