(A) Pulse-chase analysis of PCN and its deletion mutants. Cells were labeled and chased as described previously.5 The data are expressed as percent of the intracellular35S-labeled protein C present at the zero time point. (•, intracellular protein C; ○, secreted protein C). Values represent the means ± standard deviation of three independent experiments. (B) Pulse-chase analysis of wild-type protein C and its deletion mutants. Cells were labeled and chased as described previously.5 The data are expressed as percent of the intracellular35S-labeled protein C present at the zero time point (•, intracellular protein C; ○, secreted protein C; ×, total protein C, ie, the summation of secreted and intracellular material). Values represent the means ± standard deviation of three independent experiments.
