Fig. 1.
(A) The alignment of carboxyl-terminal amino acids of wtPC, PCN, and their deletion mutants. The carboxyl-terminal amino acids are indicated by standard one-letter designations. Hydrophobic regions of PCN are underlined. The hydropathy plot was performed with the LASERGENE Navigator (DNASTAR Inc, Madison, WI) determined by the methods of Kyte and Doolittle.34 (B) Northern blot analysis of the stably transfected CHO cells. Total RNA (5 μg) from each cell line was separated by 1% formaldehyde-agarose gel electrophoresis, transferred to a Zeta-Probe membrane (Bio-Rad), and hybridized with 32P-labeled PC cDNA probe.4
