American Society of Hematology

Fig. 7.

Induction of Jak2 expression in activated peripheral blood B and T lymphocytes. Human B or T cells (2 × 107cells per lane) were incubated for the indicated times with (A) 20 μg/mL anti-IgM antibody, (B) 2 mg/mL SAC, (C) 20 μg/mL anti-CD3 antibody, or (D) 10 μg/mL PHA, respectively. Cell lysates were prepared, followed by immunoprecipitation with anti-human Jak2 and immunoblotting with anti-mouse Jak2 antibody (PharMingen).

Induction of Jak2 expression in activated peripheral blood B and T lymphocytes. Human B or T cells (2 × 10⁷cells per lane) were incubated for the indicated times with (A) 20 μg/mL anti-IgM antibody, (B) 2 mg/mL SAC, (C) 20 μg/mL anti-CD3 antibody, or (D) 10 μg/mL PHA, respectively. Cell lysates were prepared, followed by immunoprecipitation with anti-human Jak2 and immunoblotting with anti-mouse Jak2 antibody (PharMingen).

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