Southern blot analysis identifying KSHV in AIDS-related NHL used as positive controls for the ISH experiments. Lanes 2, 6, 8:Hind III-digested DNA from AIDS-associated NHL containing KSHV-specific sequences identified by the KS631 probe. Lanes 3 to 5, 7, 9: AIDS-associated NHL lacking KSHV-specific sequences. Lane 1, Control DNA extracted from HL-60 promyelocytic leukemia cell line. Southern blots showing KSHV-specific sequences in the BC-1, BC-2, and BC-3 cell lines, which also were used as positive controls for ISH have been published previously.33 36