PCR-generated KSHV cyclin D probes derived from BC-3 (lanes 3 to 6). Each lane represents a PCR reaction product. Reaction products were pooled before biotinylation. Hind III-digested lambda phage DNA and Hae III-digested ◊X174 DNA mixture used as a molecular weight standard (lane 1). The Hae III-digested ◊X174 DNA was labeled and used as the irrelevant specificity probe mixture, as these fragments were of similar size to the KSHV probe. H₂O control (lane 2). PCR-generated probes were prepared and labeled as described in the text.