Fig. 4.
Dose response effect of CD34+ and CD15+/CD14− cells on stromal G-CSF production. From 10,000 to 100,000 CD34+ or CD15+/CD14− cells were plated on stromal layers. SNs were obtained on day 4 and G-CSF levels were determined by ELISA. G-CSF levels shown as percent of stromal SN is 100%. Numbers within the figure indicate number of experiments. (A) The indicated numbers of CD34+ or CD15+/CD14− cells were plated on stromal layers. Comparison between stroma and other conditions: *P < .05, ¶P < .02, ¥P < .001. (B) Combinations of CD34+ and CD15+/CD14−cells were plated together on stromal layers. Comparison between stroma and other conditions: *P < .05.