American Society of Hematology

Fig. 2.

$Fig. 2. Pharmacologic activation of c-kit stimulates cell proliferation. MTT assays for three Ba/F3 clones expressing the construct F3c-kit432. Cells were tested in the absence of IL-3 and in the presence of either FK1012 (upper panel) or an alternative dimerizer of FKBP12 domains called AP1510 (lower panel) at concentrations ranging from 10−2 nmol/L to 103 nmol/L. All Ba/F3 clones expressing F3c-kit432 showed a concentration-dependent proliferative response to both FK1012 and AP1510. Results are plotted as a fraction of OD570-630 nm values obtained using the same clone cultured in 5% WEHI conditioned medium. Data points indicate mean values of three separate experiments. Error bars indicate standard deviations. Note that in comparison to FK1012, approximately 10-fold higher concentrations of AP1510 are required to achieve equivalent proliferative effects.$

Pharmacologic activation of c-kit stimulates cell proliferation. MTT assays for three Ba/F3 clones expressing the construct F3c-kit432. Cells were tested in the absence of IL-3 and in the presence of either FK1012 (upper panel) or an alternative dimerizer of FKBP12 domains called AP1510 (lower panel) at concentrations ranging from 10⁻² nmol/L to 10³ nmol/L. All Ba/F3 clones expressing F3c-kit432 showed a concentration-dependent proliferative response to both FK1012 and AP1510. Results are plotted as a fraction of OD570-630 nm values obtained using the same clone cultured in 5% WEHI conditioned medium. Data points indicate mean values of three separate experiments. Error bars indicate standard deviations. Note that in comparison to FK1012, approximately 10-fold higher concentrations of AP1510 are required to achieve equivalent proliferative effects.

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