Blocking of CD95 expression on stimulated 185-4 cells. The 185-4 cells were cultured with either aggregated IgG (50 μg/mL) (A) or PMA (100 ng/mL) (B) in the presence of the indicated concentration of staurosporine, EGTA (1 mmol/L) or FK506 (100 nmol/L). After 24 hours, cells were stained using Jo-2 MoAb, followed by FITC-conjugated second Ab, then CD95⁺ cells were analyzed using an EPICS PROFILE flow cytometer. The results are mean ± SD of triplicate cultures.