Blocking of FcγRIII-mediated CD95 expression by FcγRII/III MoAb. The 185-4 cells were cultured in the presence of aggregated IgG (50 μg/mL) with various concentration of anti-FcγRII/III (2.4G2) MoAb. Normal rat IgG (50 μg/mL) was used as the control of 2.4G2. After 24 hours, cells were stained using Jo-2 MoAb, followed by FITC-conjugated second Ab, then CD95⁺ cells were analyzed using an EPICS PROFILE flow cytometer. The results are mean ± SD of triplicate cultures.