CD95 induction on stimulated 185-4 cells. The 185-4 cells were cultured with medium alone, aggregated IgG (50 μg/mL), A23187 (250 ng/mL), PMA (100 ng/mL), or a combination of A23187 and PMA. After 6 and 24 hours, cells were stained using anti-CD95 MoAb (Jo-2) followed by FITC-conjugated goat F(ab′) of anti-hamster IgG Ab. As the negative control of Jo-2 MoAb, cells were stained with normal hamster IgG and the CD95 negative (1) or positive (2) region was set. The percentages of CD95⁺ cells were determined using an EPICS PROFILE flow cytometer. A representative of three experiments is shown.