Fig. 3.
Downregulation of surface prion protein during in vivo granulocyte maturation. Human BM aspirates were fractionated for low-density BM cells as described. Cells were washed and stained for flow cytometry analysis as described in the legend to Fig 1. Differentiating granulocytes displaying high side scatter were gated as shown on left. As these cells mature along the granulocyte lineage and become CD15hi, there is a progressive loss of surface PrPC staining.
