American Society of Hematology

Fig. 5.

$Fig. 5. (A) Migration of CD34 cells over 16 hours at 37°C through filters coated with H120 at 15 μg/mL with IL-3 10 ng/mL, SCF 100 ng/mL, and G-CSF 500 ng/mL separately and in combination. IL-3 alone stimulated more migration than SCF (P = .01) and G-CSF (P = .01) alone. (B) Migration of CD34 cells over 16 hours at 37°C through filters coated with H120 at 15 μg/mL with different concentrations of SCF, IL-3, and G-CSF. A total of 1 ng/mL IL-3 stimulated more migration than 10 ng/mL SCF (P < .05). (C) Migration of CD34 cells from cord blood over 16 hours at 37°C through filters coated with BSA and H120 and H0 coated at 15 μg/mL with and without IL-3 10 ng/mL. Migration on H120 is increased with IL-3 (P < .005). (D) Migration of CD34 cells from cord blood over 4 hours at 37°C through filters coated with H120 at 15 μg/mL with IL-3 10 ng/mL. Effect of blocking anti-α4 and anti-β1 antibodies on IL-3 stimulated migration. Significant reduction occurred with anti-α4 (P < .01), anti-β1 (P = .01), and the combination (P = .001).$

(A) Migration of CD34 cells over 16 hours at 37°C through filters coated with H120 at 15 μg/mL with IL-3 10 ng/mL, SCF 100 ng/mL, and G-CSF 500 ng/mL separately and in combination. IL-3 alone stimulated more migration than SCF (P = .01) and G-CSF (P = .01) alone. (B) Migration of CD34 cells over 16 hours at 37°C through filters coated with H120 at 15 μg/mL with different concentrations of SCF, IL-3, and G-CSF. A total of 1 ng/mL IL-3 stimulated more migration than 10 ng/mL SCF (P < .05). (C) Migration of CD34 cells from cord blood over 16 hours at 37°C through filters coated with BSA and H120 and H0 coated at 15 μg/mL with and without IL-3 10 ng/mL. Migration on H120 is increased with IL-3 (P < .005). (D) Migration of CD34 cells from cord blood over 4 hours at 37°C through filters coated with H120 at 15 μg/mL with IL-3 10 ng/mL. Effect of blocking anti-α₄ and anti-β₁ antibodies on IL-3 stimulated migration. Significant reduction occurred with anti-α₄ (P < .01), anti-β₁ (P = .01), and the combination (P = .001).

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