Representative cytofluorographic experiment of apoptotic CD4 and CD8 cells assessed by evaluating FSC and SSC parameters. Apoptotic cells are represented as a function of both parameters. (A) Represents FSC/SSC lymphocyte compartments in an HIV-negative control and an HIV-positive individual at baseline (T0) and after 150 days of L-carnitine treatment (T4). A shift to a lower FSC/higher SSC was observed in the HIV-positive individual compared with the normal control, but L-carnitine treatment shifted FSC/SSC parameters to the levels observed in the HIV-negative control. Accordingly, CD4 and CD8 cells in the HIV-positive individual at baseline (T0) with decreased FSC were precisely those that incorporated 7-AAD (B). After 150 days of L-carnitine treatment (T4), CD4 and CD8 cells with higher FSC did not incorporate 7-AAD, as seen in the normal individual. The horizontal bar represents the threshold above which 7-AAD staining was considered positive.