Portions of human neutrophils with double labeling to identify various organelles. (a) Double labeling with sCI-MPR–biotinylated and Lactoferrin 1/1,000. The specific granules are characterized by the presence of lactoferrin (lf), with the small gold-5 nm, (arrowhead), which does not colocalize with the Man 6-P GP, as visualized with streptavidin 10 nm (*) inside the azurophilic granules. Inset: the multilaminar compartment is double labeled for the Man 6-P GP, as observed with streptavidin 10 nm (*), and LAMP-2, gold-5 nm, (arrow). (b) Double labeling with LAMP-2 (arrows). Portions of human neutrophils, which demonstrate that LAMP-2 is localized in membranes of vesicular structures, and do not colocalize with lf, the marker for specific granules. The large gold (GAM-10) labels the location of LAMP-2 in vesicles (arrows), whereas the small gold (GAR-5), (arrowheads) detects lf. (c) LAMP-2 (GAM-5), (arrows) and gelatinase (gel), (GAR-10), (arrowheads) do not colocalize either. (d) Albumin (alb) (GAR-5), (arrowheads) is found in different vesicular structures from LAMP-2 (GAM-10), (arrows).