Fig. 5.
![Fig. 5. EMSA of 32Dcl3Neo+ and 32Dcl3CDP/cut nuclear proteins bound to the DM2 LF silencer oligomers following G-CSF induction. (A) Double-stranded DM2 oligomers were [γ-32P]dATP–labeled and incubated with equal concentrations (15 μg) of nuclear extracts prepared from uninduced (lane 2) and G-CSF–induced day 2 (lane 3) and day 4 (lane 4) 32Dcl3Neo+ cells. Lanes 1 and 5, labeled DM2 oligomers without addition of any nuclear extracts. (B) [γ-32P]dATP–labeled DM2 oligomers were incubated with 15 μg/lane of nuclear extracts prepared from uninduced (lane 6), G-CSF–induced (day 2, lane 7), and G-CSF–induced (day 4, lane 8) 32Dcl3CDP/cut cells. Arrows in A and B represent the CDP/cut protein-DNA complex.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/7/10.1182_blood.v90.7.2784/3/bl_0022f5.jpeg?Expires=1723126752&Signature=PadMcII6uqeh0Li3LXk1sTeBuhE~H1lP4-0j7eI~FSKm2Ggo2F9pN6lBZ8Gt8wl6a1g5yj40ZYg7k0vlOq~4ubyv7zAG0Dj64x3cfkdbH8o-a3Tr7Lmj1s3zGRrTOTaMqgB~WxGxuFMZxUUFHwQx1sKnfGgEDY3OP7zJdTOeIiHpkFjRCFFNCYDpkKvizyEVudaIU7mKb0bYiKGJ31gu08N7Ms8igehqIAvcXPaKijDy9iQV7GFi4aS8mgTi4Sza-PmkZBxTtjmUaKYdAAPz66v60~vcE-cASIJAgb6fLGk5ieAHks~GbizV8SmXxS7jYsBZmX7v71HoZymMq3Mcig__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
EMSA of 32Dcl3Neo+ and 32Dcl3CDP/cut nuclear proteins bound to the DM2 LF silencer oligomers following G-CSF induction. (A) Double-stranded DM2 oligomers were [γ-32P]dATP–labeled and incubated with equal concentrations (15 μg) of nuclear extracts prepared from uninduced (lane 2) and G-CSF–induced day 2 (lane 3) and day 4 (lane 4) 32Dcl3Neo+ cells. Lanes 1 and 5, labeled DM2 oligomers without addition of any nuclear extracts. (B) [γ-32P]dATP–labeled DM2 oligomers were incubated with 15 μg/lane of nuclear extracts prepared from uninduced (lane 6), G-CSF–induced (day 2, lane 7), and G-CSF–induced (day 4, lane 8) 32Dcl3CDP/cut cells. Arrows in A and B represent the CDP/cut protein-DNA complex.
