Effect of culture with MDM2 antisense ODN on proliferation in RPMI 8226 MM cells. RPMI 8226 MM cells were cultured in media with MDM2 antisense ODN (A15 and A20, A and E), MDM2 sense ODN (S15 and S20, B and F ), MDM2 nonsense ODN (N15 and N20, C and G), or MDM2 scrambled ODN (X15 and X20, D and H) at concentrations of 0.1, 1.0, and 2.0 μmol/L for up to 24 hours, without replenishment of ODNs. In addition, RPMI 8226 MM cells were cultured with 1.0 μmol/L of each ODN, with replenishment of ODN every 4 hours. Proliferation was measured by 3H-TdR uptake at 0, 0.5, 1, 2, 4, 6, 8, 12, 16, 20, and 24 hours. Viable cell density at 0, 0.5, 1, 2, 4, 6, 8, 16, and 24 hours was also determined by trypan blue exclusion for RPMI 8226 MM cells cultured in 1.0 μmol/L of each ODN, without replenishment of ODN.
