Phosphorylation of paxillin and its association with RAFTK. (A) CCR5-L1.2 cells were stimulated with 100 nmol/L MIP1β for varying time intervals, and stimulated or unstimulated cell lysates were immunoprecipitated with anti-paxillin antibody. The immune precipitates were then run on SDS-PAGE and subjected to Western blotting with phosphotyrosine antibody, followed by antipaxillin and anti-RAFTK antibodies. (B) Stimulated or unstimulated cell lysates were immunoprecipitated with RAFTK antibody and blotted with paxillin antibody, followed by RAFTK antibody.