Fig. 3.
![Fig. 3. Incorporation of exogenously added GPI-anchored proteins into human erythrocytes. Erythrocytes were incubated for 1 hour at 37°C in the presence of purified [3H]-labeled AChE (A), procyclin (B), and VSG (C), and thoroughly washed to remove nonincorporated material. The total radiolabel in the wash supernatants was counted and used to calculate the amounts of label incorporated into erythrocytes. The figure shows the relative incorporation of intact (bars a), GPI-PLD–treated (bars b), mild base-treated (bars c), and Pronase-treated (bars d) GPI-anchored proteins. The numbers represent the mean values ± SD from n independent experiments with the number of experiments shown in parentheses. *, Not determined.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/5/10.1182_blood.v91.5.1784/3/blod4052603.jpeg?Expires=1724260317&Signature=beK30-FMq1QN7uLknBPwfL58ARGzitauVfs21JHu4ni1S9qKGn8mw0SKKofnsbdpWDQprPosO1FhaaeWWB~R0DB6jjmmC4dNmEAqsx5EhaEgK5KUhcnFWPKSBYV3Vf3v2PmjXaKG1BLgvXQuYqixq5Xvg0sAAPnoGRa--osoLbWjHk8bw7hzgMekD~5vbrfErO3tj4OrEy5ebnc3umUPy5xvFQW66VaRiz0mj0yTyNtZcUmPEyWOpwvwdTVvA-Ugd1zMl2-OUx~LAM7Zfb4jolbEreelyFxLnRwo6EbG2ibwk7baQ-kdXo4sb-hMJokGpnxHlNEb9G3m2pqRMUnRwg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Incorporation of exogenously added GPI-anchored proteins into human erythrocytes. Erythrocytes were incubated for 1 hour at 37°C in the presence of purified [3H]-labeled AChE (A), procyclin (B), and VSG (C), and thoroughly washed to remove nonincorporated material. The total radiolabel in the wash supernatants was counted and used to calculate the amounts of label incorporated into erythrocytes. The figure shows the relative incorporation of intact (bars a), GPI-PLD–treated (bars b), mild base-treated (bars c), and Pronase-treated (bars d) GPI-anchored proteins. The numbers represent the mean values ± SD from n independent experiments with the number of experiments shown in parentheses. *, Not determined.
