Expression of the IL-2R by cells from blood collected with different anticoagulants. (A) Blood of one donor was collected on CPD and PBMC were isolated the same day as the blood sample. Staining and analysis of CD4 T lymphocytes was performed as indicated in Fig 1except that FL3-labeled anti-CD14 MoAb was used. (B) Blood was collected on sodique heparin (n = 9) or on CPD (n = 3) or on ACD (n = 3), and PBMC were isolated the same day as the blood sample (d = 0) or 1 day after (d = 1) in some cases with sodique heparin (n = 4). Staining and analysis was performed as in (A) for CD4 T lymphocytes and as indicated in Fig 4 for CD8 T lymphocytes. Significant differences are indicated as follows, a, P ≤ .0001; b, .0001 < P ≤ .001; c, .001 < P ≤ .01; and d, .01 < P < .05 (nonpaired t-test). (C) Blood was collected on sodique heparin (H) or on CPD (C). CD4 T lymphocytes and monocytes were purified from PBMC isolated at d=0. IL-2Rα and β specific mRNAs were measured as previously described.30HPRT detection is also shown as positive control; N, PCR negative control.