![Fig. 2. HIV-1 infection of primary human stromal layers. Primary human stromal layers were infected with HIV-1JR-FL at MOI of 1.0 and 0.1 in duplicate. On day 8 after infection, human monocytic cells were added to one of each pair of cultures to amplify low levels of HIV-1 present. Samples of the culture supernatants were sampled every 4 days and analyzed for p24 gag protein by ELISA. (A) Shows the results of four independent infections of stromal layers with HIV-1 at an MOI = 1 without subsequent addition of monocytic cells (error bars represent the SEM). (B) Shows the results of four independent infections of stromal layers with HIV-1 at an MOI = 1 (the duplicates from [A]), with monocytic cells added on day 8. (C) Shows the results of four independent infections of stromal layers at an MOI = 0.1 without subsequent addition of monocytic cells. (D) Shows the results of four independent infections of stromal layers with HIV-1 at an MOI = 0.1 (the duplicates from [C]) with monocytic cells added on day 8.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/5/10.1182_blood.v90.5.1787/4/bl_0040f2a.jpeg?Expires=1722700442&Signature=NtHDhdjWS1l0bWq2PfqCCanE-c0d4gMVdJNwC-4YbGSVsr6yb~dVFOEoKLYCXMsWnThyPiFcxG2DRJox7Nu63sZCA5zfYqPThffHnDovFYA19PvmZQpOiMztZ6tbUoske6ZkE9OJ0jbr4IGrdWQTo2eBaaBlnTpDZxJ9rFiwZCRRRCNq7CM~VzWd9ZPdTvXlr2rOkG2HrwpaxQ8HUrHg~J2F1biGLEeeVq~-fObuPX54W-koyvdEFN9paIPs6660DfDVM3A63c4DV-geUIFBDYEQAJ0vIKKP3Kc5ZtT83a-QGgsL5-Q-ssrmAbwT~WRb4pPWyd15HKFmDyHzmcnJ4w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HIV-1 infection of primary human stromal layers. Primary human stromal layers were infected with HIV-1JR-FL at MOI of 1.0 and 0.1 in duplicate. On day 8 after infection, human monocytic cells were added to one of each pair of cultures to amplify low levels of HIV-1 present. Samples of the culture supernatants were sampled every 4 days and analyzed for p24 gag protein by ELISA. (A) Shows the results of four independent infections of stromal layers with HIV-1 at an MOI = 1 without subsequent addition of monocytic cells (error bars represent the SEM). (B) Shows the results of four independent infections of stromal layers with HIV-1 at an MOI = 1 (the duplicates from [A]), with monocytic cells added on day 8. (C) Shows the results of four independent infections of stromal layers at an MOI = 0.1 without subsequent addition of monocytic cells. (D) Shows the results of four independent infections of stromal layers with HIV-1 at an MOI = 0.1 (the duplicates from [C]) with monocytic cells added on day 8.
