American Society of Hematology

Fig. 3.

$Fig. 3. Separation of thrombin digest of rTFPI on reverse phase-HPLC. (A) The 9.5 hours digest of rTFPI with thrombin was applied to a COSMOSIL 5C18-300 column that had been equilibrated with 0.05% trifluoroacetic acid. The fragments were eluted with 0.05% trifluoroacetic acid containing a linear concentration gradient of acetonitrile from 36% to 54%. (B) SDS-PAGE of each fraction, peak I, peak II, and peak III from reversed phase HPLC. See text for nomenclature of band 1, band 2, and band 3.$

Separation of thrombin digest of rTFPI on reverse phase-HPLC. (A) The 9.5 hours digest of rTFPI with thrombin was applied to a COSMOSIL 5C18-300 column that had been equilibrated with 0.05% trifluoroacetic acid. The fragments were eluted with 0.05% trifluoroacetic acid containing a linear concentration gradient of acetonitrile from 36% to 54%. (B) SDS-PAGE of each fraction, peak I, peak II, and peak III from reversed phase HPLC. See text for nomenclature of band 1, band 2, and band 3.

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