Fig. 2.
![Fig. 2. GM-CSF signals for proliferation in LNCaP cells. (A) Growth curve. LNCaP cells were maintained in continuous culture with no stimulation and the cell number was determined by counting the cells every day for 6 days and the cell viability was assessed by exclusion of trypan blue. (B) Time course. Cells were incubated with 0.3 (•) or 100 nmol/L (○) GM-CSF for 1 to 6 days and parallel cultures were pulse-labeled with [3H]-thymidine for 20 hours every day. (C) Dose response. Cells were incubated with increased amounts of GM-CSF (0.01 to 100 nmol/L) for 3 (•) or 4 days (○) and pulse-labeled with [3H]-thymidine for the last 20 hours.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/3/10.1182_blood.v91.3.1037/3/blod4032602.jpeg?Expires=1722776043&Signature=gaRfbJm7D1BaOHeWvATzTd-vO4Ky~TvAaTWpD~TaTUdlPe9FAoVN5u-xVLI0FTxO7CcfPtx525Xb6gG4IPLsTkUzSbN0D1ROqSL3uuBVlbNrd-8L9eiyLNNDr0nS0-3s12iyIuQMSoZ2o9z2jupRW8-j7K1rwHr9COzPYYCASfbzud~Vi0l0Hohq~Vf8GQMx2l0f4qVBl9fW3Jw2yWXmDKaulihlzeMfMIhwcKaLnGf5tgUPBjdMywFDF7NW0KeBczJPFz3~G9O4UzM9DA4BPkHjHO1CYBJ6oLWFuOZhvL3N-cikXZZ-98bDvtyJTCy6AO7g-LO93mW40usyxyTKIw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
GM-CSF signals for proliferation in LNCaP cells. (A) Growth curve. LNCaP cells were maintained in continuous culture with no stimulation and the cell number was determined by counting the cells every day for 6 days and the cell viability was assessed by exclusion of trypan blue. (B) Time course. Cells were incubated with 0.3 (•) or 100 nmol/L (○) GM-CSF for 1 to 6 days and parallel cultures were pulse-labeled with [3H]-thymidine for 20 hours every day. (C) Dose response. Cells were incubated with increased amounts of GM-CSF (0.01 to 100 nmol/L) for 3 (•) or 4 days (○) and pulse-labeled with [3H]-thymidine for the last 20 hours.
