Fig. 3.
![Fig. 3. Exposure of P-selectin on the surface of single platelets during adhesion to fibrinogen or collagen matrices. Fura-2–loaded platelets were allowed to adhere to immobilized fibrinogen (A) or collagen (B) in the presence of 2 mmol/L CaCl2 and FITC-labeled AK-6 antibody against P-selectin. Arrows indicate timepoint of addition of 2 nmol/L thrombin or timepoint of attachment to collagen. Fluorescence images were taken during 5 to 10 minutes, and traces represent changes in FITC fluorescence of individual platelets. These platelets were high in [Ca2+]i at the end of the FITC fluorescence recordings. Traces are representative of >35 platelets out of three independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/7/10.1182_blood.v90.7.2615/3/bl_0025f3.jpeg?Expires=1722619914&Signature=vT0tUCLpLUuYcxdKL-SaBMtQV6RGie49s4CaN9LCrAp~gNi8bFT247vyxH3ww8IfcZWMNxeyOUuwE-K~gt0a6syL1jtsSzSbKMfV54-IEJaiRBgvDDXAx~W-cnovsNcHJu-KoCWQb6ISU2EX2KSmyRhMTWu4rGDfEgudpEXTE4QQdF~miyFChgTQ4OziBHv6C7FHeeZAD6u4Kq~MFm5cVgM6mpyqMiw5PW5pXTMs5joGrK9DyZHfx0o8EB60gIISeYVnRvNq55Ox-M-A2M6sPl6g6YoPDpgRwm0sRKlF172QkRJsqHaAPvSAXJefbO9SO~B6mXbR17dS3rcMjhmFlQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Exposure of P-selectin on the surface of single platelets during adhesion to fibrinogen or collagen matrices. Fura-2–loaded platelets were allowed to adhere to immobilized fibrinogen (A) or collagen (B) in the presence of 2 mmol/L CaCl2 and FITC-labeled AK-6 antibody against P-selectin. Arrows indicate timepoint of addition of 2 nmol/L thrombin or timepoint of attachment to collagen. Fluorescence images were taken during 5 to 10 minutes, and traces represent changes in FITC fluorescence of individual platelets. These platelets were high in [Ca2+]i at the end of the FITC fluorescence recordings. Traces are representative of >35 platelets out of three independent experiments.
