American Society of Hematology

Fig. 4.

$Fig. 4. Human EBV+ B-cell tumors from hu-PBL-SCID mice and from patients with AIDS-PCNSL are susceptible to Fas-induced apoptosis. Quantitative assessment of apoptosis was obtained by staining cell nuclei with PI and analyzing 5,000 events by flow cytometry.30 Tumor cells plated in the presence of 10 μg/mL of immobilized anti-Fas M3 MoAb and a fivefold excess of soluble M3 have only 17% of nuclei within the hypodiploid portion of the histogram, indicative of apoptosis (A). Tumor cells plated in the presence of 10 μg/mL of immobilized anti-Fas M3 MoAb alone have 45% of nuclei within the apoptotic portion of the histogram (B). Similar results were obtained with tumor cells from a fresh AIDS-PCNSL biopsy specimen in that only 19% of nuclei were within the hypodiploid portion after exposure to 10 μg/mL of immobilized M3 MoAb with an excess of soluble M3 (C), while 57% were within the apoptotic portion after exposure to 10 μg/mL of immobilized M3 MoAb alone (D). Similar results were obtained when LCLs derived from hu-PBL-SCID B-cell tumors were plated in the presence of 5 μg/mL of the mobilized anti-Fas IgM CH11 MoAb. Twenty-one percent of the cells were in the apoptotic fraction after exposure to an IgM isotype control MoAb (E), while 73% of cells were within the apoptotic fraction after exposure to mobilized anti-Fas IgM CH11 MoAb (F).$

Human EBV⁺ B-cell tumors from hu-PBL-SCID mice and from patients with AIDS-PCNSL are susceptible to Fas-induced apoptosis. Quantitative assessment of apoptosis was obtained by staining cell nuclei with PI and analyzing 5,000 events by flow cytometry.30 Tumor cells plated in the presence of 10 μg/mL of immobilized anti-Fas M3 MoAb and a fivefold excess of soluble M3 have only 17% of nuclei within the hypodiploid portion of the histogram, indicative of apoptosis (A). Tumor cells plated in the presence of 10 μg/mL of immobilized anti-Fas M3 MoAb alone have 45% of nuclei within the apoptotic portion of the histogram (B). Similar results were obtained with tumor cells from a fresh AIDS-PCNSL biopsy specimen in that only 19% of nuclei were within the hypodiploid portion after exposure to 10 μg/mL of immobilized M3 MoAb with an excess of soluble M3 (C), while 57% were within the apoptotic portion after exposure to 10 μg/mL of immobilized M3 MoAb alone (D). Similar results were obtained when LCLs derived from hu-PBL-SCID B-cell tumors were plated in the presence of 5 μg/mL of the mobilized anti-Fas IgM CH11 MoAb. Twenty-one percent of the cells were in the apoptotic fraction after exposure to an IgM isotype control MoAb (E), while 73% of cells were within the apoptotic fraction after exposure to mobilized anti-Fas IgM CH11 MoAb (F).

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