MLR-stimulatory capacity of generated cells. Purified CD34⁺ CB cells were cultured for 10 days in serum-free medium in the presence of TGF-β1, GM-CSF, TNFα, and SCF with or without FL (see the Materials and Methods). Graded numbers of cells obtained from these cultures were used to stimulate 5 × 10⁴ purified allogeneic T cells (see the Materials and Methods) and were compared with adult PBMNC. Data are representative of five experiments.