Fig. 4.
(A) Growth kinetics of parental and retrovirus-infected 32D cells in G-CSF–supplemented culture medium. Cells were seeded at 2.5 × 105 cells/mL; viable cells were determined by trypan blue dye exclusion test. (B) Morphology of parental, 32D/LXSN, and 32D/PU.1 cells in the presence of IL-3 or after 3, 5, and 10 days of stimulation with G-CSF. Cells were removed from the cultures, cytocentrifuged onto slides, air-dried, and stained with May-Grünwald-Giemsa.
