American Society of Hematology

Fig. 5.

$Fig. 5. PCR detection of IgH rearrangement. Four representative results from unprocessed and processed BM and PBPC are shown. Left upper panel: Data from patient 25 (persistance of the IgH rearrangement in the CD34+ cell fraction of BM). Right upper panel: Data from patients 33 (negativity of the IgH rearrangement in the three fractions) and 37 (persistence of the IgH rearrangement in the CD34+ cell fraction of PBPC). Lower panel: Data from patient 41 (disappearance of the IgH rearrangement in the CD34+ cell fraction of PBPC). Control+, B-ALL cells; Control−, normal PB lymphocytes; BC, DNA extracted from buffy coat (unprocessed BM or unprocessed PBPC); CD34−, DNA extracted from the unadsorbed CD34− cell fraction; CD34+, DNA extracted from the adsorbed CD34+ cell fraction; MW, molecular weight markers. Arrows show the specific abnormal JH rearranged band.$

PCR detection of IgH rearrangement. Four representative results from unprocessed and processed BM and PBPC are shown. Left upper panel: Data from patient 25 (persistance of the IgH rearrangement in the CD34⁺ cell fraction of BM). Right upper panel: Data from patients 33 (negativity of the IgH rearrangement in the three fractions) and 37 (persistence of the IgH rearrangement in the CD34⁺ cell fraction of PBPC). Lower panel: Data from patient 41 (disappearance of the IgH rearrangement in the CD34⁺ cell fraction of PBPC). Control⁺, B-ALL cells; Control⁻, normal PB lymphocytes; BC, DNA extracted from buffy coat (unprocessed BM or unprocessed PBPC); CD34⁻, DNA extracted from the unadsorbed CD34⁻ cell fraction; CD34⁺, DNA extracted from the adsorbed CD34⁺ cell fraction; MW, molecular weight markers. Arrows show the specific abnormal JH rearranged band.

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