Intracellular localization of the 15-LOX in human eosinophilic granulocytes. Cell preparation and immunogold labeling was performed as described in the Materials and Methods section. (A) Unstimulated eosinophilic granulocyte (12,700-fold magnification); (B) unstimulated eosinophilic granulocyte (30,400-fold magnification); (C) eosinophilic granulocyte stimulated with 4 μmol/L calcium ionophore A 23187 (29,600-fold magnification).